Plasmid

Part:BBa_K530039:Design

Designed by: Daniel Bibl   Group: iGEM11_Johns_Hopkins   (2011-09-24)


pRSBB426 URA3 2Micron Episomal Yeast Shuttle Vector


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 5186
    Illegal PstI site found at 395
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 395
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 5186
    Illegal PstI site found at 395
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 5186
    Illegal PstI site found at 395
    Illegal NgoMIV site found at 1676
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1085
    Illegal BsaI.rc site found at 3356
    Illegal SapI site found at 2273
    Illegal SapI.rc site found at 932


Design Notes

QuickChange PCR


Source

pRS 426

References